Anti-Human IL4 Recombinant Antibody (TAB-221)

Name: Anti-Human IL4 Recombinant Antibody (TAB-221)
SKU: TAB-221
Rating: 5 (1 reviews)

CAT#: TAB-221

Recombinant monoclonal antibody to IL4. It is a humanized monoclonal antibody for the treatment of asthma.

Tested Data Gene Expression

ELISA

Figure 1 Anti-Human IL4 Recombinant Antibody (TAB-221) in ELISA

ELISA analysis of TAB-221 was performed by coating with human IL4 protein (His tag). Then blocked with BSA and incubated with TAB-221. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human IL4 Recombinant Antibody (TAB-221) in WB

Western blot analysis of TAB-221 was performed by loading human IL4 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-221 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-Human IL4 Recombinant Antibody (TAB-221) in DB

Dot Blot analysis of TAB-221 was performed by coating with human IL4 protein (His tag).
TAB-221 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

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Normal Tissue Figure 1 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78477/168090_A_5_6.jpg

RNA Expression Figure 2 RNA cell line category: Cell line enriched (MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113520-IL4

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Mouse

Derivation
Humanized (from mouse)

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
WB, ELISA, FC, IP, FuncS, IF, Neut

Related Disease
Allergy

Product Property

Purity
>95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The IL4 antibody has been reported in applications of Inhib, Neut.

Target

Alternative Names
Pascolizumab;331243-22-2;SB-240683;IL4;interleukin 4;interleukin-4;B cell growth factor 1;B_cell stimulatory factor 1;BCGF 1;BCGF1;BSF1;IL 4;lymphocyte stimulatory factor 1;MGC79402;binetrakin;pitrakinra;IL-4;BSF-1;BCGF-1;

Gene ID
3565

UniProt ID
P05112

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(Creative Biolabs Cat# TAB-221, RRID: AB_3111881)

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Biosimilar Overview

Please refer to Pascolizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Pascolizumab.

Related Diseases

Asthma

Autoimmune Thyroid Disease

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-221.pdf

MSDS

MSDS - TAB-221.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
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TAB-667LC-F(E)	Human Anti-IL4 Recombinant Antibody; Fab Fragment (TAB-667LC-F(E))	ELISA, FC	Human Fab

CAT	Product Name	Application	Type
NEUT-1403CQ	Rat Anti-IL4 Recombinant Antibody (clone 25D2), Biotin	Block, ELISA, IHC, WB	Rat IgG1
NEUT-1420CQ	Rat Anti-Il4 Recombinant Antibody (NEUT-1420CQ)	Block, DB	Rat IgG1
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CAT	Product Name	Application	Type
NEUT-1424CQ	Rat Anti-Il4 Recombinant Antibody (clone CBL238)	ELISA, Neut	Rat IgG2b
NEUT-1425CQ	Rat Anti-Il4 Recombinant Antibody (clone CBL899)	ELISA, FC, IF, Neut, WB	Rat IgG2b, κ
NEUT-1426CQ	Rat Anti-Il4 Recombinant Antibody (clone CBL993)	Neut, ELISA	Rat IgG1
NEUT-1427CQ	Rat Anti-Il4 Recombinant Antibody (clone 11B11)	ELISA, CyTOF, ELISPOT, Neut, ICC, IP, IHC	Rat IgG1, κ
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CAT	Product Name	Application	Type
MOR-1812	Rabbit Anti-IL4 Recombinant Antibody (clone DS1812AB)	ICC, WB	Rabbit IgG
MOR-4273	Rabbit Anti-IL4 Recombinant Antibody (clone SI356DS)	ELISA	Rabbit IgG
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CAT	Product Name	Application	Type
HPAB-0079-YC-S(P)	Mouse Anti-IL4 Recombinant Antibody (clone m3B9); scFv Fragment	ELISA	Mouse scFv
HPAB-0080-YC-S(P)	Human Anti-IL4 Recombinant Antibody (clone hu3B9); scFv Fragment	ELISA	Humanized scFv
HPAB-0081-YC-S(P)	Mouse Anti-IL4 Recombinant Antibody (clone m19C11); scFv Fragment	Block, FuncS	Mouse scFv
HPAB-0082-YC-S(P)	Human Anti-IL4 Recombinant Antibody (clone hu19C11); scFv Fragment	ELISA	Humanized scFv
HPAB-1537WJ-S(P)	Human Anti-IL4 Recombinant Antibody; scFv Fragment (clone h25D2-9)	ELISA, WB, Neut	Human scFv

CAT	Product Name	Application	Type
HPAB-0079-YC-F(E)	Mouse Anti-IL4 Recombinant Antibody (clone m3B9); Fab Fragment	ELISA	Mouse Fab
HPAB-0080-YC-F(E)	Human Anti-IL4 Recombinant Antibody (clone hu3B9); Fab Fragment	ELISA	Humanized Fab
HPAB-0081-YC-F(E)	Mouse Anti-IL4 Recombinant Antibody (clone m19C11); Fab Fragment	Block, FuncS	Mouse Fab
HPAB-0082-YC-F(E)	Human Anti-IL4 Recombinant Antibody (clone hu19C11); Fab Fragment	ELISA	Humanized Fab
HPAB-1537WJ-F(E)	Human Anti-IL4 Recombinant Antibody; Fab Fragment (clone h25D2-9)	ELISA, WB, Neut	Human Fab

CAT	Product Name	Application	Type
MOB-085LC	Mouse Anti-IL4 Recombinant Antibody (clone YC133)	ELISA, FuncS	Mouse IgG
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ZG-0746F	Rat Anti-IL4 Antibody (clone AR.98)	FC, IF	Rat IgG1
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VS13-YC582	Rabbit Anti-IL4 Recombinant Antibody (VS13-YC582)	WB	Rabbit IgG

CAT	Product Name	Application	Type
AFC-TAB-221	Afuco™ Anti-IL4 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-221)	ELISA, FC, IP, FuncS, IF	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS3-WK1322	Mouse Anti-IL4 Recombinant Antibody (VS3-WK1322)	ELISA, WB	Mouse IgM

CAT	Product Name	Application	Type
VS-0225-XY141	CytoStream™ Rat Anti-IL4 Recombinant Antibody (clone 11B11)	FC	Rat IgG1
VS-0225-XY142	CytoStream™ Mouse Anti-IL4 Recombinant Antibody (VS-0225-XY142)	ELISA, FC	Mouse IgG1, kappa
VS-0225-XY143	CytoStream™ Rat Anti-IL4 Recombinant Antibody (VS-0225-XY143)	ELISA, FC, Neut, WB	Rat IgG2b

CAT	Product Name	Application	Type
VS-0425-FY131	Human Anti-IL4 scFv-Fc Chimera (VS-0425-FY131)	ELISA, Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0525-XY3531	Anti-Mouse IL4 Immunohistochemistry Kit	IHC

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