Anti-Human IL4 Recombinant Antibody (TAB-221)

Name: Anti-Human IL4 Recombinant Antibody (TAB-221)
SKU: TAB-221
Rating: 5 (1 reviews)

CAT#: TAB-221

Recombinant monoclonal antibody to IL4. It is a humanized monoclonal antibody for the treatment of asthma.

Tested Data Gene Expression

ELISA

Figure 1 Anti-Human IL4 Recombinant Antibody (TAB-221) in ELISA

ELISA analysis of TAB-221 was performed by coating with human IL4 protein (His tag). Then blocked with BSA and incubated with TAB-221. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human IL4 Recombinant Antibody (TAB-221) in WB

Western blot analysis of TAB-221 was performed by loading human IL4 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-221 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-Human IL4 Recombinant Antibody (TAB-221) in DB

Dot Blot analysis of TAB-221 was performed by coating with human IL4 protein (His tag).
TAB-221 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

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Normal Tissue Figure 1 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78477/168090_A_5_6.jpg

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Figure 2 RNA cell line category: Cell line enriched (MOLT-4)

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Mouse

Derivation
Humanized (from mouse)

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
WB, ELISA, FC, IP, FuncS, IF, Neut

Related Disease
Allergy

Product Property

Purity
>95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The IL4 antibody has been reported in applications of Inhib, Neut.

Target

Alternative Names
Pascolizumab;331243-22-2;SB-240683;IL4;interleukin 4;interleukin-4;B cell growth factor 1;B_cell stimulatory factor 1;BCGF 1;BCGF1;BSF1;IL 4;lymphocyte stimulatory factor 1;MGC79402;binetrakin;pitrakinra;IL-4;BSF-1;BCGF-1;

Gene ID
3565

UniProt ID
P05112

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(Creative Biolabs Cat# TAB-221, RRID: AB_3111881)

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Biosimilar Overview

Please refer to Pascolizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Pascolizumab.

Related Diseases

Asthma

Autoimmune Thyroid Disease

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-221.pdf

MSDS

MSDS - TAB-221.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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